In the Image Processing Basics section on
Calibrating
Images we discussed only one type of image calibration -- dark
subtraction. This is the most important calibration step, but not the only
one that can be performed. Other techniques include using flat fields and
bias frames to calibrate a raw CCD image.
Flat Fields
Flat fields are used to remove variations in the brightness of pixels across
the CCD chip. These variations are usually caused either by variances in
sensitivity from pixel to pixel, or by the telescope optics. Main causes
due to the telescope are vignetting and
out-of-focus dust on one of the lenses in the optical system.
Below is an uncalibrated image of the Whirlpool Galaxy.

Below is a flat field taken using the identical imaging system.

Select the proper flat field file of files in the Calibrate window as shown
below. Notice that multiple dark frames have also been selected as you will want to
perform a dark subtract as well. (See the Image Processing Basics section
on Calibrating Images for more details on dark
subtracting an image.) In this case, using several calibration images
reduces the noise in those images.

Above: Calibration window set up to median combine three darks and
three flats. The flats will also be dark subtracted.
Calibrating the image should result in an image similar to the one
below. Note that the noise (white specks) in the image have been removed
by the dark frame, and that the uneven background illumination and dust halos
have been eliminated by the flat field.

Above: The calibrated image of M51. Combining the
images would be the next step to remove the grainy appearance.
Bias Frames
As pixels are read out of a CCD camera there is a slight offset, or bias,
that occurs. This offset varies from pixel to pixel and can cause noise in
the image. A bias frame is used to subtract this offset.
A bias frame is an exposure of zero duration. When this exposure is
read out the only information contained is the bias values and a slight amount
of read noise from the process of transferring the charge out of the CCD.
Bias is inherent in dark frames as well as light frames (including flats).
So, as long as you dark subtract your lights and flats, there is no need to take
bias frames. If you are scaling dark frames (which usually is not
recommended) then you will need separate bias frames.
Next, Combining Images....

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